diff --git a/modules/nrf_wifi/os/CMakeLists.txt b/modules/nrf_wifi/os/CMakeLists.txt
index daef05f2098..a409fb81023 100644
--- a/modules/nrf_wifi/os/CMakeLists.txt
+++ b/modules/nrf_wifi/os/CMakeLists.txt
@@ -44,7 +44,6 @@ target_compile_definitions(
   NRF70_RX_MAX_DATA_SIZE=${CONFIG_NRF70_RX_MAX_DATA_SIZE}
   NRF70_MAX_TX_PENDING_QLEN=${CONFIG_NRF70_MAX_TX_PENDING_QLEN}
   NRF70_RPU_PS_IDLE_TIMEOUT_MS=${CONFIG_NRF70_RPU_PS_IDLE_TIMEOUT_MS}
-  NRF70_REG_DOMAIN=${CONFIG_NRF70_REG_DOMAIN}
   NRF70_BAND_2G_LOWER_EDGE_BACKOFF_DSSS=${CONFIG_NRF70_BAND_2G_LOWER_EDGE_BACKOFF_DSSS}
   NRF70_BAND_2G_LOWER_EDGE_BACKOFF_HT=${CONFIG_NRF70_BAND_2G_LOWER_EDGE_BACKOFF_HT}
   NRF70_BAND_2G_LOWER_EDGE_BACKOFF_HE=${CONFIG_NRF70_BAND_2G_LOWER_EDGE_BACKOFF_HE}