diff --git a/tests/bluetooth/addr/testcase.yaml b/tests/bluetooth/addr/testcase.yaml
index 54cfe092aee..7726b736be9 100644
--- a/tests/bluetooth/addr/testcase.yaml
+++ b/tests/bluetooth/addr/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
diff --git a/tests/bluetooth/bluetooth/testcase.yaml b/tests/bluetooth/bluetooth/testcase.yaml
index 3c54ea6f7bf..ccdd504c763 100644
--- a/tests/bluetooth/bluetooth/testcase.yaml
+++ b/tests/bluetooth/bluetooth/testcase.yaml
@@ -5,8 +5,6 @@ tests:
     platform_allow:
       - qemu_x86
       - qemu_cortex_m3
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
     integration_platforms:
diff --git a/tests/bluetooth/bt_crypto/testcase.yaml b/tests/bluetooth/bt_crypto/testcase.yaml
index ba933462590..a9cef7cd404 100644
--- a/tests/bluetooth/bt_crypto/testcase.yaml
+++ b/tests/bluetooth/bt_crypto/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
@@ -17,8 +15,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
diff --git a/tests/bluetooth/bt_crypto_ccm/testcase.yaml b/tests/bluetooth/bt_crypto_ccm/testcase.yaml
index 77dd0dcbb8e..859cfea2abe 100644
--- a/tests/bluetooth/bt_crypto_ccm/testcase.yaml
+++ b/tests/bluetooth/bt_crypto_ccm/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
     integration_platforms:
diff --git a/tests/bluetooth/controller/ctrl_isoal/testcase.yaml b/tests/bluetooth/controller/ctrl_isoal/testcase.yaml
index d638f089b3d..63b03aff2d0 100644
--- a/tests/bluetooth/controller/ctrl_isoal/testcase.yaml
+++ b/tests/bluetooth/controller/ctrl_isoal/testcase.yaml
@@ -3,7 +3,6 @@ common:
 tests:
   bluetooth.isoal.test:
     platform_allow:
-      - native_posix
       - native_sim
     integration_platforms:
       - native_sim
diff --git a/tests/bluetooth/controller/ctrl_sw_privacy_unit/testcase.yaml b/tests/bluetooth/controller/ctrl_sw_privacy_unit/testcase.yaml
index 7dd0dcbc16e..190e57a14ec 100644
--- a/tests/bluetooth/controller/ctrl_sw_privacy_unit/testcase.yaml
+++ b/tests/bluetooth/controller/ctrl_sw_privacy_unit/testcase.yaml
@@ -3,7 +3,6 @@ common:
 tests:
   bluetooth.privacy.test:
     platform_allow:
-      - native_posix
       - native_sim
     integration_platforms:
       - native_sim
diff --git a/tests/bluetooth/gatt/testcase.yaml b/tests/bluetooth/gatt/testcase.yaml
index fd349d00f52..37e14f0b968 100644
--- a/tests/bluetooth/gatt/testcase.yaml
+++ b/tests/bluetooth/gatt/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
@@ -19,8 +17,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
diff --git a/tests/bluetooth/hci_prop_evt/testcase.yaml b/tests/bluetooth/hci_prop_evt/testcase.yaml
index e1a41372ada..260afaaf589 100644
--- a/tests/bluetooth/hci_prop_evt/testcase.yaml
+++ b/tests/bluetooth/hci_prop_evt/testcase.yaml
@@ -5,8 +5,6 @@ tests:
     platform_allow:
       - qemu_x86
       - qemu_cortex_m3
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
     integration_platforms:
diff --git a/tests/bluetooth/hci_uart_async/testcase.yaml b/tests/bluetooth/hci_uart_async/testcase.yaml
index b189149d104..f62852ce5c1 100644
--- a/tests/bluetooth/hci_uart_async/testcase.yaml
+++ b/tests/bluetooth/hci_uart_async/testcase.yaml
@@ -7,7 +7,6 @@ tests:
       - uart
     harness: ztest
     platform_allow:
-      - native_posix
       - native_sim
     integration_platforms:
       - native_sim
diff --git a/tests/bluetooth/host_long_adv_recv/testcase.yaml b/tests/bluetooth/host_long_adv_recv/testcase.yaml
index dfe4465a42e..a3acd20de15 100644
--- a/tests/bluetooth/host_long_adv_recv/testcase.yaml
+++ b/tests/bluetooth/host_long_adv_recv/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
     integration_platforms:
diff --git a/tests/bluetooth/l2cap/testcase.yaml b/tests/bluetooth/l2cap/testcase.yaml
index 5f19cf2eb73..285b4ff8128 100644
--- a/tests/bluetooth/l2cap/testcase.yaml
+++ b/tests/bluetooth/l2cap/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86
diff --git a/tests/bluetooth/mesh/blob_io_flash/testcase.yaml b/tests/bluetooth/mesh/blob_io_flash/testcase.yaml
index d5ad661d9ef..869709f9b16 100644
--- a/tests/bluetooth/mesh/blob_io_flash/testcase.yaml
+++ b/tests/bluetooth/mesh/blob_io_flash/testcase.yaml
@@ -3,7 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
       - native_sim
     tags:
       - bluetooth
diff --git a/tests/bluetooth/mesh/delayable_msg/testcase.yaml b/tests/bluetooth/mesh/delayable_msg/testcase.yaml
index 5b5e9ee0f37..63a008dfc56 100644
--- a/tests/bluetooth/mesh/delayable_msg/testcase.yaml
+++ b/tests/bluetooth/mesh/delayable_msg/testcase.yaml
@@ -1,7 +1,6 @@
 tests:
   bluetooth.mesh.delayable_msg:
     platform_allow:
-      - native_posix
       - native_sim
     tags:
       - bluetooth
diff --git a/tests/bluetooth/mesh/rpl/testcase.yaml b/tests/bluetooth/mesh/rpl/testcase.yaml
index 16b947fe626..c00cf451cbf 100644
--- a/tests/bluetooth/mesh/rpl/testcase.yaml
+++ b/tests/bluetooth/mesh/rpl/testcase.yaml
@@ -1,7 +1,6 @@
 tests:
   bluetooth.mesh.rpl:
     platform_allow:
-      - native_posix
       - native_sim
     tags:
       - bluetooth
diff --git a/tests/bluetooth/tester/boards/native_posix.conf b/tests/bluetooth/tester/boards/native_posix.conf
deleted file mode 100644
index 30abfdbc3d9..00000000000
--- a/tests/bluetooth/tester/boards/native_posix.conf
+++ /dev/null
@@ -1,3 +0,0 @@
-CONFIG_UART_PIPE=n
-CONFIG_SERIAL=y
-CONFIG_UART_NATIVE_POSIX=y
diff --git a/tests/bluetooth/tester/testcase.yaml b/tests/bluetooth/tester/testcase.yaml
index 93868152f7d..5af4a957fae 100644
--- a/tests/bluetooth/tester/testcase.yaml
+++ b/tests/bluetooth/tester/testcase.yaml
@@ -3,7 +3,6 @@ tests:
     build_only: true
     platform_allow:
       - qemu_x86
-      - native_posix
       - native_sim
       - nrf52840dk/nrf52840
     tags: bluetooth
@@ -21,7 +20,6 @@ tests:
     build_only: true
     platform_allow:
       - qemu_x86
-      - native_posix
       - native_sim
       - nrf5340dk/nrf5340/cpuapp
     extra_args: OVERLAY_CONFIG="overlay-le-audio.conf"
@@ -31,7 +29,6 @@ tests:
     build_only: true
     platform_allow:
       - qemu_x86
-      - native_posix
       - native_sim
       - nrf52840dk/nrf52840
     extra_args: OVERLAY_CONFIG="overlay-mesh.conf"
diff --git a/tests/bluetooth/uuid/testcase.yaml b/tests/bluetooth/uuid/testcase.yaml
index e3382e875c9..59627c936c7 100644
--- a/tests/bluetooth/uuid/testcase.yaml
+++ b/tests/bluetooth/uuid/testcase.yaml
@@ -3,8 +3,6 @@ tests:
     extra_args:
       - EXTRA_DTC_OVERLAY_FILE="test.overlay"
     platform_allow:
-      - native_posix
-      - native_posix/native/64
       - native_sim
       - native_sim/native/64
       - qemu_x86