diff --git a/boards/st/nucleo_wb09ke/arduino_r3_connector.dtsi b/boards/st/nucleo_wb09ke/arduino_r3_connector.dtsi
index 75fb046f29f..7b61215ab79 100644
--- a/boards/st/nucleo_wb09ke/arduino_r3_connector.dtsi
+++ b/boards/st/nucleo_wb09ke/arduino_r3_connector.dtsi
@@ -25,4 +25,6 @@
 	};
 };
 
+arduino_i2c: &i2c1 {};
 arduino_serial: &usart1 {};
+arduino_spi: &spi3 {};
diff --git a/boards/st/nucleo_wb09ke/nucleo_wb09ke.dts b/boards/st/nucleo_wb09ke/nucleo_wb09ke.dts
index 733e6c0f717..1052c04dd94 100644
--- a/boards/st/nucleo_wb09ke/nucleo_wb09ke.dts
+++ b/boards/st/nucleo_wb09ke/nucleo_wb09ke.dts
@@ -101,3 +101,18 @@
 	current-speed = <115200>;
 	status = "okay";
 };
+
+&i2c1 {
+	pinctrl-0 = <&i2c1_scl_pb6 &i2c1_sda_pb7>;
+	pinctrl-names = "default";
+	status = "okay";
+};
+
+&spi3 {
+	pinctrl-0 = <&spi3_nss_pa9 &spi3_sck_pb3 &spi3_miso_pa8 &spi3_mosi_pa11>;
+	pinctrl-names = "default";
+	status = "okay";
+	/* Select 64MHz clock for SPI3 */
+	clocks = <&rcc STM32_CLOCK_BUS_APB1 (1 << 14)>,
+		<&rcc STM32_SRC_SYSCLK SPI3_I2S3_SEL(3)>;
+};
diff --git a/boards/st/nucleo_wb09ke/nucleo_wb09ke.yaml b/boards/st/nucleo_wb09ke/nucleo_wb09ke.yaml
index f9931787abb..c645659d26a 100644
--- a/boards/st/nucleo_wb09ke/nucleo_wb09ke.yaml
+++ b/boards/st/nucleo_wb09ke/nucleo_wb09ke.yaml
@@ -9,5 +9,9 @@ toolchain:
 ram: 64
 flash: 512
 supported:
+  - arduino_i2c
+  - arduino_spi
   - gpio
+  - i2c
+  - spi
 vendor: st